ANALYSIS OF CHROMOSOMAL DNA CONTENT IN PACIFIC RED ABALONE HALIOTIS RUFESCENS BY FLUORESCENCE IMAGE ANALYSIS

2005 ◽  
Vol 24 (4) ◽  
pp. 1161-1168 ◽  
Keyword(s):  
Image Analysis ◽  
Dna Content ◽  
Chromosomal Dna ◽  
Fluorescence Image ◽  
Haliotis Rufescens ◽  
Red Abalone ◽  
Chromosomal Dna Content

Mean nuclear area and chromosomal DNA content of squamous cell carcinomas of the oral cavity using computerized image analysis

1997 ◽  
Vol 111 (2) ◽  
pp. 141-144 ◽  
Author(s):  
S. K. Sarker ◽  
K. S. Patel
Keyword(s):  
Image Analysis ◽  
Oral Cavity ◽  
Dna Content ◽  
Squamous Cell Carcinomas ◽  
Nuclear Area ◽  
Chromosomal Dna ◽  
Node Negative ◽  
Computerized Image Analysis ◽  
Node Positive ◽  
Chromosomal Dna Content

AbstractMorphometric measurements of nuclei may be of prognostic value in some cancers. In this present study we have evaluated the mean nuclear area (MNA) of 50 squamous cell carcinomas of the oral cavity (SCCOC) using computerized image analysis. Since chromosomal DNA content is a reflection of the DNA content in the nucleus, we have evaluated the relationship between MNA and chromosomal DNA. Thirteen tumours had a MNA greater than 49.9 μm2 and 37 had a MNA less than this. Six tumours were classified as hypodiploid, 29 as diploid and 15 as aneuploid. There were 44 node-negative patients and six node-positive. When comparing MNA in these groups, 50 per cent of node-positive patients had a larger MNA whilst only 20 per cent of the node-negative group had a large MNA. The correlation coefficient between MNA and DNA indices was r = 0.75. The greater nuclear size is possibly a reflection of a more aggressive tumour biology in the node-positive patients. We conclude that a large MNA may be a marker of aggressive tumour biology in this group. In the future, we aim to evaluate the prognostic significance of MNA in patients with SCCOC.

scholarly journals Comparison of the Coffea canephora and C. arabica karyotype based on chromosomal DNA content

2008 ◽  
Vol 28 (1) ◽  
pp. 73-81 ◽  
Author(s):  
Wellington Ronildo Clarindo ◽  
Carlos Roberto Carvalho
Keyword(s):  
Dna Content ◽  
Coffea Canephora ◽  
Chromosomal Dna ◽  
Chromosomal Dna Content

scholarly journals Chromosomal DNA content of sweet pepper determined by association of cytogenetic and cytometric tools

2008 ◽  
Vol 27 (7) ◽  
pp. 1227-1233 ◽  
Author(s):  
Isabella Santiago de Abreu ◽  
Carlos Roberto Carvalho ◽  
Wellington Ronildo Clarindo
Keyword(s):  
Dna Content ◽  
Sweet Pepper ◽  
Chromosomal Dna ◽  
Chromosomal Dna Content

Bivariate flow karyotyping of human chromosomes: Evaluation of variation in Hoechst 33258 fluorescence, chromomycin A3 fluorescence, and relative chromosomal DNA content

Cytometry ◽  
1991 ◽  
Vol 12 (6) ◽  
pp. 559-569 ◽  
Author(s):  
Gert A. Boschman ◽  
Wim Rens ◽  
Carel H. van Oven ◽  
Erik M. M. Manders ◽  
Jacob A. Aten
Keyword(s):  
Dna Content ◽  
Human Chromosomes ◽  
Hoechst 33258 ◽  
Chromosomal Dna ◽  
Chromomycin A3 ◽  
Chromosomal Dna Content

scholarly journals Genotoxicity Assessment of Potassium Bromate by Means of DNA Image Analysis on the Root Tip Nuclei of Allium sativum L.

2020 ◽  
pp. 9-14
Author(s):  
Hoda, A. Khatab ◽  
Nagat, S. Elhaddad ◽  
Samia Eissa
Keyword(s):  
Image Analysis ◽  
Dna Content ◽  
Allium Sativum ◽  
Food Additives ◽  
Genotoxic Effect ◽  
Root Tip ◽  
Dependent Manner ◽  
Chromosomal Dna ◽  
Efficient Test ◽  
Dna Image Analysis

Allium sativum assay was widely used to assess the compound's cytotoxicity and genotoxicity on plants and animals. Only few studies analyzed the genotoxic effect of potassium bromated (KBrO3) on the DNA content of plant cells. DNA content assay is an efficient test for the measure of chromosomal DNA damages. Based on this approach the effect of KBrO3 on DNA content change was investigated in root tip nuclei of A. sativum. Different concentrations of food additives KBrO3. 3 g/l, 5 g/l 7 g/l and 9 g/l % were prepared and treatments were given. A. sativum root were incubated for 2, 6 and 24 hours and DNA image analysis of root tip nuclei was performed. The analysis was based on the measurement of the Mean Optical Density (MOD) which represents the cellular DNA content. The results showed that the KBrO3 significantly decreased of DNA content compared to the control at all concentrations and treatment periods in dose-dependent manner. The present study suggests that extensive use of food additives should be banned due to genotoxic effect on living cells. Therefore, there is an urgent need to evaluate potential mutagenic effects of KBrO3 on human.

scholarly journals An improved, non-isotopic method of screening cells from patients with abnormalities of sexual differentiation for Y chromosomal DNA content.

1993 ◽  
Vol 30 (4) ◽  
pp. 304-307 ◽  
Author(s):  
M Witt ◽  
K Michalczak ◽  
A Latos-Bielenska ◽  
J Jaruzelska ◽  
I Kuczora ◽  
...  
Keyword(s):  
Dna Content ◽  
Sexual Differentiation ◽  
Chromosomal Dna ◽  
Isotopic Method ◽  
Chromosomal Dna Content

DECREASE IN CHROMOSOMAL DNA CONTENT AT HIGHER POLYPLOIDY LEVEL IN VICIA FABA ROOT MERISTEMS

1973 ◽  
Vol 15 (4) ◽  
pp. 863-865 ◽  
Author(s):  
P. C. Deka ◽  
S. K. Sen
Keyword(s):  
Vicia Faba ◽  
Ploidy Level ◽  
Dna Content ◽  
Nuclear Dna ◽  
Root Meristem ◽  
Nuclear Dna Content ◽  
Chromosomal Dna ◽  
Root Meristems ◽  
Chromosomal Dna Content

The DNA content of the nucleus is expected to increase proportionately with the increase of ploidy. Estimation of the DNA content of the endopolyploid root meristem nuclei of Vicia faba has been done through cytophotometry from 2n to 16n level. Observations indicate that a decline in nuclear DNA content takes place at higher ploidy level while the overall karyological integrity of the chromosomes and their number are apparently maintained as normal.

Monitoring protease activity in human sputum samples by FRET-based probes via statistics-based fluorescence image analysis

Pneumologie ◽  
2015 ◽  
Vol 69 (07) ◽  
Author(s):  
F Stein ◽  
N Heath ◽  
S Dittrich ◽  
MA Mall ◽  
C Schultz
Keyword(s):  
Image Analysis ◽  
Protease Activity ◽  
Fluorescence Image

Improved Assessment of Isolated Islet Tissue Volume Using Digital Image Analysis

1998 ◽  
Vol 7 (5) ◽  
pp. 469-478 ◽  
Author(s):  
Jan P. Stegemann ◽  
John J. O'Neil ◽  
Don T. Nicholson ◽  
Claudy J.-P. Mullon
Keyword(s):  
Image Analysis ◽  
Digital Image ◽  
Dna Content ◽  
Digital Image Analysis ◽  
Volume Measurement ◽  
Volume Determination ◽  
Tissue Volume ◽  
Porcine Islet ◽  
Islet Volume ◽  
Islet Tissue

Accurate and consistent measurement of tissue volume is critical to performing many types of islet research; however, conventional visual determination of isolated islet yields through a microscope is heavily operator dependent. An improved method of islet volume determination using digital image analysis (DIA) was developed to remove operator bias and automate the islet counting process. A series of 140 porcine islet isolations were used to evaluate the DIA method in three separate stages. In Stage 1 ( n = 29 isolations), the conventional and DIA methods were correlated with two other independent islet quantitation methods: insulin extraction, and DNA extraction. It was found that volumes determined by DIA correlated more closely with insulin content and DNA content than did conventionally determined volumes. In Stages 2 and 3 ( n = 54 and 57 isolations, respectively), it was shown that an increase in the number of fields analyzed by DIA did not significantly improve the quality of the correlations. Inclusion of very small tissue (<50 fun in diameter), which is ignored in the conventional protocol affected yields by less than 10% and did not significantly improve the correlation with insulin or DNA content. Quantitation of isolated islet tissue volume using DIA has been shown to be rapid, consistent, and objective. In the laboratory, use of this method as the standard for islet volume measurement will allow more meaningful comparison of experimental results between centers. In the clinic, its use will allow more accurate dosing of transplanted tissue. © 1998 Elsevier Science Inc.

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